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ionizable lipid alc 0315  (MedChemExpress)


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    MedChemExpress ionizable lipid alc 0315
    Ionizable Lipid Alc 0315, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ionizable+lipid+alc+0315/ALC-0315/pmc13010108-272-6-9
    Average 97 stars, based on 70 article reviews
    ionizable lipid alc 0315 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Transmission Assay:

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: Bioinspired Virus-Like Porous Silica Amplify Lipid-Mediated mRNA Delivery
    Article Snippet: LPs were prepared using thin-film hydration method.LPs were prepared using thin-film hydration method.. First, the stock solutions of cationic lipids, 1,2-Dioleoyl-3-trimethylammonium-propane chloride (DOTAP, MedChemExpress, USA) and 1,2-di-O-octadecenyl-3-trimethylammonuim propane (DOTMA, Indagoo, Europe), ionizable lipid ALC-0315 (MedChemExpress, USA), and helper lipids, DOPE (MedChemExpress, USA) and DSPC (MedChemExpress, USA) were prepared in chloroform.. The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: 571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid 576 mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), 577 cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine 578 (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid 579 ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-580 0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: A comprehensive investigation of Glycoprotein-based nucleic acid vaccines for Hantaan Virus
    Article Snippet: (LNP) formulations were prepared using a modified procedure, previously employed for siRNA , . .. In summary, lipids, including ionizable lipid (ALC-0315) (MCE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (MCE), cholesterol (MCE) and PEG-lipid (MCE), were dissolved in ethanol at a molar ratio of 55:10:32.5:2.5. .. The 10 mM lipid mixture was mixed with 10 mM citrate buffer containing DNA at a 1:3 ratio through a mixer.

    Electron Microscopy:

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: Bioinspired Virus-Like Porous Silica Amplify Lipid-Mediated mRNA Delivery
    Article Snippet: LPs were prepared using thin-film hydration method.LPs were prepared using thin-film hydration method.. First, the stock solutions of cationic lipids, 1,2-Dioleoyl-3-trimethylammonium-propane chloride (DOTAP, MedChemExpress, USA) and 1,2-di-O-octadecenyl-3-trimethylammonuim propane (DOTMA, Indagoo, Europe), ionizable lipid ALC-0315 (MedChemExpress, USA), and helper lipids, DOPE (MedChemExpress, USA) and DSPC (MedChemExpress, USA) were prepared in chloroform.. The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: 571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid 576 mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), 577 cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine 578 (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid 579 ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-580 0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: A comprehensive investigation of Glycoprotein-based nucleic acid vaccines for Hantaan Virus
    Article Snippet: (LNP) formulations were prepared using a modified procedure, previously employed for siRNA , . .. In summary, lipids, including ionizable lipid (ALC-0315) (MCE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (MCE), cholesterol (MCE) and PEG-lipid (MCE), were dissolved in ethanol at a molar ratio of 55:10:32.5:2.5. .. The 10 mM lipid mixture was mixed with 10 mM citrate buffer containing DNA at a 1:3 ratio through a mixer.

    Modification:

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: Bioinspired Virus-Like Porous Silica Amplify Lipid-Mediated mRNA Delivery
    Article Snippet: LPs were prepared using thin-film hydration method.LPs were prepared using thin-film hydration method.. First, the stock solutions of cationic lipids, 1,2-Dioleoyl-3-trimethylammonium-propane chloride (DOTAP, MedChemExpress, USA) and 1,2-di-O-octadecenyl-3-trimethylammonuim propane (DOTMA, Indagoo, Europe), ionizable lipid ALC-0315 (MedChemExpress, USA), and helper lipids, DOPE (MedChemExpress, USA) and DSPC (MedChemExpress, USA) were prepared in chloroform.. The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: 571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid 576 mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), 577 cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine 578 (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid 579 ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-580 0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: A comprehensive investigation of Glycoprotein-based nucleic acid vaccines for Hantaan Virus
    Article Snippet: (LNP) formulations were prepared using a modified procedure, previously employed for siRNA , . .. In summary, lipids, including ionizable lipid (ALC-0315) (MCE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (MCE), cholesterol (MCE) and PEG-lipid (MCE), were dissolved in ethanol at a molar ratio of 55:10:32.5:2.5. .. The 10 mM lipid mixture was mixed with 10 mM citrate buffer containing DNA at a 1:3 ratio through a mixer.

    Transfection:

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: Bioinspired Virus-Like Porous Silica Amplify Lipid-Mediated mRNA Delivery
    Article Snippet: LPs were prepared using thin-film hydration method.LPs were prepared using thin-film hydration method.. First, the stock solutions of cationic lipids, 1,2-Dioleoyl-3-trimethylammonium-propane chloride (DOTAP, MedChemExpress, USA) and 1,2-di-O-octadecenyl-3-trimethylammonuim propane (DOTMA, Indagoo, Europe), ionizable lipid ALC-0315 (MedChemExpress, USA), and helper lipids, DOPE (MedChemExpress, USA) and DSPC (MedChemExpress, USA) were prepared in chloroform.. The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: 571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid 576 mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), 577 cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine 578 (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid 579 ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-580 0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: A comprehensive investigation of Glycoprotein-based nucleic acid vaccines for Hantaan Virus
    Article Snippet: (LNP) formulations were prepared using a modified procedure, previously employed for siRNA , . .. In summary, lipids, including ionizable lipid (ALC-0315) (MCE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (MCE), cholesterol (MCE) and PEG-lipid (MCE), were dissolved in ethanol at a molar ratio of 55:10:32.5:2.5. .. The 10 mM lipid mixture was mixed with 10 mM citrate buffer containing DNA at a 1:3 ratio through a mixer.

    Fluorescence:

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).mRNA-LNP formulation for animal experiments was performed using the NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: Bioinspired Virus-Like Porous Silica Amplify Lipid-Mediated mRNA Delivery
    Article Snippet: LPs were prepared using thin-film hydration method.LPs were prepared using thin-film hydration method.. First, the stock solutions of cationic lipids, 1,2-Dioleoyl-3-trimethylammonium-propane chloride (DOTAP, MedChemExpress, USA) and 1,2-di-O-octadecenyl-3-trimethylammonuim propane (DOTMA, Indagoo, Europe), ionizable lipid ALC-0315 (MedChemExpress, USA), and helper lipids, DOPE (MedChemExpress, USA) and DSPC (MedChemExpress, USA) were prepared in chloroform.. The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.The third helper lipid, DOPC (Avanti Research, USA) was originally in chloroform at the concentration of 25 mg/ml.

    Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines
    Article Snippet: 571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).571 572 573 Jo urn al Pr e-p roo f 24 mRNA-LNP vaccine formulation 574 mRNA-lipid nanoparticle (LNP) formulation for animal experiments was performed using the 575 NanoAssemblr Spark system (Precision Nanosystems, Vancouver, Canada).. Briefly, a lipid 576 mixture consisting of ionizable lipid ALC-0315 (MedChemExpress; cat. no. HY-138170), 577 cholesterol (Sigma-Aldrich; cat. no. C8667), helper lipid distearoylphosphatidylcholine 578 (DSPC) (Avanti Polar Lipids, Alabaster, AL, USA; cat. no. 85030), and PEG-conjugated lipid 579 ALC-0159 (MedChemExpress; cat. no. HY-138300) was prepared at a molar ratio of ALC-580 0315:DSPC:cholesterol:ALC-0159 = 46.3:9.4:42.7:1.6.. The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.The lipid mixture was combined with 581 mRNA in citrate buffer (pH 4) at a nitrogen-to-phosphate (N:P) ratio of 6:1.

    Article Title: A comprehensive investigation of Glycoprotein-based nucleic acid vaccines for Hantaan Virus
    Article Snippet: (LNP) formulations were prepared using a modified procedure, previously employed for siRNA , . .. In summary, lipids, including ionizable lipid (ALC-0315) (MCE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (MCE), cholesterol (MCE) and PEG-lipid (MCE), were dissolved in ethanol at a molar ratio of 55:10:32.5:2.5. .. The 10 mM lipid mixture was mixed with 10 mM citrate buffer containing DNA at a 1:3 ratio through a mixer.



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